Destruxin A inhibits the hemocytin-mediated hemolymph immunity of host insects to facilitate Metarhizium infection.

Insects have an effective innate immune system to protect themselves against fungal invasion. Metarhizium employs a toxin-based strategy using a nonribosomal peptide called destruxin A (DA) to counteract the host immune response. However, the mechanism by which DA inhibits insect immunity is still unclear. Here, we identified 48 DA-binding proteins in silkworm hemolymph, with the binding affinity (KD) ranging from 2 to 420 µM. Among these proteins, hemocytin, an important immune factor, was determined to be the strongest DA-binding protein. DA binds to hemocytin and regulates its conformation in a multisite manner. Furthermore, DA exerts a significant inhibitory effect on hemocytin-mediated hemocyte aggregation. By disrupting the interaction between hemocytin, actin A3, and gelsolin, DA prevents the transformation of granules into vesicles in hemocytes. These vesicles are responsible for storing, maturing, and exocytosing hemocytin. Therefore, hemocytin secretion is reduced, and the formation of structures that promote aggregation in outer hemocytes is inhibited.

Hemocytin, the special aggregation factor connecting insect hemolymph immunity, a potential target of insecticidal immunosuppresant.

Insects possess an effective innate immunity that enables them to adapt to their intricate living environment and fend off various pathogens (or parasites). This innate immunity comprises both humoral and cellular immunity, which synergistically orchestrate immune responses. Hemocytin, a lectin with a distinctive structure, plays a crucial role in insect hemolymph immunity. Hemocytin is involved in the early immune response, facilitating processes such as coagulation, nodulation, and encapsulation in the hemolymph. It prevents hemolymph overflow and microbial pathogens invasion resulting from epidermal damage, and also aids in the recognition and elimination of invaders. However, the research on hemocytin is still limited. Our previous findings demonstrated that destruxin A effectively inhibits insect hemolymph immunity by interacting with hemocytin, suggesting that hemocytin could be a potential target for insecticides development. Therefore, it is crucial to gain a deeper understanding of hemocytin. This review integrates recent advancements in the study of the structure and function of insect hemocytin and also explores the potential of hemocytin as a target for insecticides. This review aims to enhance our comprehension of insect innate immunity and provide innovative ideas for the development of environmentally friendly pesticides.

Mechanism of destruxin a inhibits juvenile hormone binding protein transporting juvenile hormone to affect insect growth.

Destruxin A, a non-ribosomal peptide toxin produced by Metarhizium, exhibits potent insecticidal activity by targeting various tissues, organs, and cells of insects. Our previous research has revealed that DA possesses the ability to bind to multiple proteins. In this study, we aimed to identify the most sensitive binding proteins of DA and investigate the physiological processes in which DA regulated. Through RNAi technology, we screened 22 binding proteins of DA in silkworm hemolymph. Among them, the juvenile hormone binding protein (JHBP), a hormone transport protein crucial for growth and development regulation, exhibited the highest sensitivity to DA. Subsequent experiments demonstrated that DA could inhibit the body weight gain of silkworm larvae, accelerate the pupation occurrence, and modulate the content of free juvenile hormone (JH) in the hemolymph. We also observed that DA could induce conformational changes in both the JHBP and the JHBP-JH binding complex. Notably, at low dosage, DA influenced the binding of JHBP to JH, while at high dosage, it irreversibly affected the binding of JHBP to JH. Molecular docking and point-mutant experiments suggested that DA might affect the N-arm of JHBP, which is responsible for JH binding. Additionally, we discovered that JHBP is widely distributed in various tissues of the silkworm, including the epidermis, gut, fat body, Malpighian tubule, gonad, muscle, trachea, and hemocyte. This study provides novel insights into the insecticidal mechanism of DA and enhances our understanding of the pathogenic process of Metarhizium.